Workplace Health and Safety for Newsletters- myassignmenthelp

Question: Talk about theWorkplace Health and Safety for Industry Newsletters. Answer: Raising WHS issues In the current task, the Spudshed store had been mulled over. It is an Australia based general store taking into account the new food prerequisites of individuals (Home - Spudshed, 2017). The task gives a reasonable diagram of the job of the work environment wellbeing and security (WHS) officials in executing adequate preventive measures. One of the urgent advances which I can take here are featuring and raising the WHS issues looked by my representatives throughout their work. A portion of the overwhelming WHS issues are lifting and conveying substantial loads alongside the danger of slips, excursions and falls. Spudshed being a food store the staffs need to convey and ship quintals of new products of the soil (McCaughey et al., 2017). This can bring about unexpected muscle injuries or nerve tissue harm in the staff. Furthermore, confined conditions and wet floors can likewise bring about fall danger of the staffs. In this manner, as a WHS official, I have to sort out gatherings for the individuals and various partners of the association and impart to them the specific hazard factors included. I likewise need to give a fake arrangement to the board individuals in regards to the approaches to lessen mishaps inside the work environment. A portion of these may incorporate fuse of more streetcars with wheels for pulling and uprooting the heavier burdens (Arbury et al., 2017). Assembling and imparting data about wellbeing and security issues So as to examine with my staff about appropriate WHS measures and approaches, I can follow various sources. A portion of these sources are government acts or distributions, industry bulletins, ex-workers alongside director supervisors of the grocery store. As a rehearsing WHS official, I can execute various devices and procedures for discovering the significant security situation of the current grocery store. In this specific situation, I can utilize talk with techniques as pertinent instruments for social affair adequate information about the security mediations gave to the representatives in the spudshed general store. There are various WHS issues which are looked by the representatives working in a grocery store. Along these lines, as a rehearsing WHS official, I have to actualize various preventive and careful steps for the security and sound soundness of the staffs. The WHS data can be conveyed to the laborers utilizing various mediation techniques and conventions. I as a WHS official can lead various worker preparing programs inside the concerned general store. As I would see it giving the representatives adequate security instruction and innovation based strategies can change their method of work and diminish the hazard rate. References Arbury, S., Zankowski, D., Lipscomb, J., Hodgson, M. (2017). Work environment brutality preparing programs for social insurance laborers: an investigation of program elements.Workplace Health Safety,65(6), 266-272. McCaughey, D., Halbesleben, J. R., Savage, G. T., Simons, T., McGhan, G. E. (2014). Security authority: Extending work environment wellbeing atmosphere best practices across human services workforces. InLeading in medicinal services associations: Improving security, fulfillment and money related performance(pp. 189-217). New Jersey : Emerald Group Publishing Limited. Zohar, D. (2014). Wellbeing atmosphere: Conceptualization, estimation, and improvement.The Oxford handbook of authoritative atmosphere and culture, 317-334. home - Spudshed. (2017). Spudshed. Recovered 21 October 2017, from https://www.spudshed.com.au/

The United States of America Essay Example for Free

The United States of America Essay It is 2039 in the United States of America. There is another compulsory government law and strategy that requires every American resident to hold fast to. The method is known as the Ein which is a German word that implies â€Å"one†. The technique changes all typical individuals as we probably am aware them into hyper-savvy individuals and reproduces them into excellent creatures. The procedure begins a programmed lift on which the person rides, and goes starting with one machine then onto the next. The primary machine controls the mind by utilizing a programmed electronic head piece that drops from above onto the human head. The electronic headpiece stuns the cerebrum and reinvents everything that is thoughtful, for example, feelings, sympathy and imagination. After that piece of the cerebrum is totally desensitized, the PC programming starts to change the brain, transforming each and every individual who goes into distanced, egocentric people. After the procedure is done, the human is completely stunned and naturally moves to the following machine which is known as the Van-Trans. To shield the human from feeling any agony, the person is first infused with a super torment reliever and afterward the human’s skeleton reshapes into a human box. After the individual is formed like a container, the Van-Trans start the reshaping strategy. In the wake of embellishment and cutting, the individual is made into a supermodel structure. This is a legislative standard necessity for all individuals to follow who lives inside the United States. The entirety of the people who are reproduced are alluded to as Supers by the administration. At last, when the methodology is done, the Super is currently permitted to leave the Machine. On one event in the wake of having the system done, there was a young lady who observes a young lady who was harmed in an auto crash and she required the youthful woman’s help. The young lady was oblivious and nobody would help her in light of how the necessary strategy influences each and every individual who has the method done. The entirety of their feelings and sympathy is evacuated during the strategy. Subsequently, the lady just ventured directly over the youngster having no inclination at all for the young lady. The world has been transformed into a coldblooded society. The legislature has made a general public that is numb to having sentiments and loves their mind and external man. â€Å"Power has become so unpretentious and complex a thing [†¦] that lone an inconspicuous psyche can watch it work; here it is as yet constrained, still visible† (LeGuin 5). The people’s just force is their insight however they utilize their insight to get over on other people who are not Supers like them. â€Å"Nothing succeeds like success† (LeGuin 5). Works Cited LeGuin, Ursula K. The Left Hand Of Darkness. New York: Walker And Company, 1969.

Wireless Communication

Remote Communication Free Online Research Papers Remote interchanges are all over the place. Individuals around the globe normally send and get messages remotely, that is, transmitted through the air. Three kinds of remote correspondences incorporate remote informing administrations, remote web passageways, and worldwide situating frameworks. Individuals utilize cell phones, PDAs and other cell phones to get to content informing, texting, and picture informing administrations (Davies 34-42). Through content informing administrations, clients send and get short instant messages, which as a rule comprise of less than 300 characters. Remote texting is an Internet correspondences administration that permits a remote cell phone to trade texts that at least one cell phones or online PCs. Clients send designs, pictures, video cut, sound records, and short instant messages with picture informing administrations. In numerous open areas, individuals interface with the Internet through a remote Internet passageway utilizing versatile PC and gadgets. Two kinds of remote Internet passageways are problem areas and 3G systems. A problem area is a remote system that permits versatile clients to check email, peruse the Web, and access any Internet administration †as long as their PCs or gadgets have the suitable remote ability. A 3G organize, which utilizes cell radio innovation, empowers clients to interface with the Internet through a cell phone or PC furnished with a proper PC card. A worldwide situating framework (GPS) is a route framework that comprises of at least one earth-based beneficiaries that acknowledge and examine signals sent by satellites so as to decide the receiver’s geographic area, as per Shelly and Cashman (How GPS Works). A GPS collector is a handheld, mountable, or implanted gadget that contains a reception apparatus, a radio beneficiary, and a processor. Numerous cell phones, for example, cell phones and PDAs, have GPS ability incorporated with the gadget. Portable clients convey remotely through remote informing administrations, remote Internet passages, and worldwide situating frameworks. Anybody can exploit remote correspondences utilizing portable PCs and gadgets. ? Davies, Habika. Content Messaging, Instant Messaging, and Picture Messaging Services. Figuring in Todays World January 2008: 34-42. Podpora, Maxine C., and Adlbert D. Ruiz. Advances in Wireless Internet Access Point Technology. Dallas: Wells Publishing , 2008. Shelly, Gary B., and Thomas J. Cashman. How GPS Works. Course Technology. 21 March 2008 . Research Papers on Wireless CommunicationBionic Assembly System: A New Concept of SelfPETSTEL investigation of IndiaAnalysis of Ebay Expanding into AsiaThe Project Managment Office SystemOpen Architechture a white paperRiordan Manufacturing Production PlanRelationship between Media Coverage and Social andAnalysis Of A Cosmetics AdvertisementInfluences of Socio-Economic Status of Married MalesNever Been Kicked Out of a Place This Nice

2019 Best Brightest Brandon Kunick, University of Richmond (Robins)

Brandon Kunick 2019 Best Brightest: Brandon Kunick, University of Richmond (Robins) by: Jeff Schmitt on March 23, 2019 | 0 Comments Comments 267 Views March 23, 2019Brandon KunickUniversity of Richmond, Robins School of Businessâ€Å"I learned everything from my golden retriever: how to play, relax, and do my business.†Fun fact about yourself: I have found over 300 four-leaf clovers in my lifetime.Hometown: Victor, NYHigh School: Victor Senior High SchoolMajor: Accounting and FrenchMinor: N/AFavorite Business Course: Strategic ManagementExtracurricular Activities, Community Work and Leadership Roles During College: Vice President of Finance for the Robins School of Business Student Government Association; Tour guide; Future American Men of Excellence (FAME); Theta Chi Fraternity; Varsity Lacrosse; Club Lacrosse, Vice President of Badminton Club; Boatwright Scholar; Phi Eta Sigma honors society; Omicron Delta Kappa Leadership Society; Academic Excellence medal for highest GPA on the Men ’s Lacrosse team.Where have you interned during your college career? Mengel Metzger Barr Co LLP – Audit intern, Rochester, NYKPMG – Global Audit intern, Richmond, VA and Kuala Lumpur, MYWhere will you be working after graduation? KPMG, Richmond office – Audit AssociateWhat company do you admire most? I really enjoy the business model of Warby Parker. I learned about the company in high school. Even though I don’t wear glasses, if I did, I would definitely choose to wear theirs.Who is your favorite professor? It is so hard to choose since I have had so many amazing professors here, but Professor Joe Ben Hoyle made a huge impact on me here at the University of Richmond. His class was my first ever class here and that class is largely what inspired me to choose accounting as my major.What did you enjoy most about your business school? I love the size of the classes. I get to know the professors really well and I can also meet most of my peers as the small class sizes help facilitate conversations.What advice would you give to a student looking to major in a business-related field? There are so many opportunities in business so don’t just settle for an area of study because it’s the easy option. Find a subject you are passionate about and pursue it with passion.â€Å"If I didn’t major in business, I would be majoring in or studying†¦International Business.†Who most influenced your decision to pursue business in college? My interest in business actually just came from a coin collection I got from my grandfather when I was young. I got into coin collecting and figured out I wanted to work with money the rest of my life, though I wasn’t sure quite how I wanted to do that.Which academic, extracurricular or personal achievement are you most proud of? I walked onto the varsity lacrosse team here at Richmond my sophomore year. It was an amazing experience and I always wanted to play lacrosse in c ollege, so it felt amazing accomplishing that goal.Which classmate do you most admire? I really admire Cole Hurford. I have had a few classes with Cole, and he has grown to be a great friend of mine here at school. He has been in charge of several different student organizations including new student orientation and president of the business school. He has handled each of these leadership positions exceptionally well and I am impressed that he has been able to manage being a part of so many organizations while still excelling at school and other extracurricular activitiesWho would you most want to thank for your success?   My parents have been huge role models for me throughout my life. They have both done incredible things and have inspired me to constantly be the best I can possibly be. So many of my values have come from them and I would love to thank them for that.What are the top two items on your bucket list? Jump into the top 10 rooftop pools in the world (I’ve alrea dy been in two). Visit all seven continentsWhat are your hobbies? Lacrosse, badminton, coin collectingWhat made Brandon such an invaluable addition to the Class of 2019â€Å"Brandon Kunick is one of the Robins School’s best and brightest students because of his enthusiasm and wide range of interests. I met Brandon when he enrolled in my first-year seminar Higher Education: From Aristotle to Animal House. In the seminar, we discussed the various goals of a liberal arts education including improving writing skills, appreciating art, building character, broadening one’s interests, and preparing for citizenship. Brandon was interested in everything. It was clear that he was not in college just to get a job.Three years later, Brandon enrolled in my 400-level Auditing class. One of the pleasures of teaching both freshmen and seniors is seeing their development. Brandon had grown into a confident, articulate, serious student while losing none of his enthusiasm. From his seat in the front row, he led class discussions and earned some of the highest exam scores in the class.Besides academics, Brandon’s interests range from music to athletics. He played saxophone in the UR jazz band and made the varsity lacrosse team as a walk-on. His future employer, KPMG, is fortunate Brandon will be joining them in the fall. I’m delighted that Brandon accepted a job in their Richmond office where he will be able to visit campus often for recruiting and alumni events.†Dr. Paul M. Clikeman Associate Professor of Accounting Page 1 of 11

The Imjin War, Japanese Invasions of Korea

Dates: May 23, 1592 - December 24, 1598 Adversaries:  Japan versus Joseon Korea and Ming China Troop strength:   Korea - 172,000 national army and navy, 20,000 insurgent fighters Ming China - 43,000 imperial troops (1592 deployment); 75,000 to 90,000 (1597 deployment) Japan - 158,000 samurai and sailors (1592 invasion); 141,000 samurai and sailors (1597 invasion) Outcome:  Victory for Korea and China, led by Korean naval successes. Defeat for Japan. In 1592, the Japanese warlord Toyotomi Hideyoshi launched his samurai armies against the Korean Peninsula. It was the opening move in the Imjin War (1592-98). Hideyoshi envisioned this as the first step in a campaign to conquer Ming China; he expected to roll over Korea quickly, and even dreamed of going on to India once China had fallen. However, the invasion did not go as Hideyoshi planned. Build-up to the First Invasion    As early as 1577, Toyotomi Hideyoshi wrote in a letter that he had dreams of conquering China. At the time, he was just one of Oda Nobunagas generals. Japan itself was still in the throes of the Sengoku or Warring States period, a century-long era of chaos and civil war among the different domains. By 1591, Nobunaga was dead and Hideyoshi was in charge of a much more unified Japan, with northern Honshu the last major region to fall to his armies.   Having accomplished so much, Hideyoshi began to give serious thought once more to his old dream of taking on China, the major power of East Asia.   A victory would prove the might of reunified Japan, and bring her immense glory. Hideyoshi first sent emissaries to the court of Joseon Koreas King Seonjo in 1591, requesting permission to send a Japanese army through Korea on its way to attack China. The Korean king refused.   Korea had long been a tributary state of Ming China, while relations with Sengoku Japan had seriously deteriorated thanks to incessant Japanese pirate attacks all along Koreas coast.   There was simply no way that the Koreans would allow Japanese troops to use their country as a staging ground for an assault on China. King Seonjo sent his own embassies to Japan in turn, to try and learn what Hideyoshis intentions were. The different ambassadors returned with different reports, and Seonjo chose to believe those who said that Japan would not attack. He made no military preparations. Hideyoshi, however, was busy gathering an army of 225,000 men. Its officers and most of the troops were samurai, both mounted and foot soldiers, under the leadership of some major daimyo from Japans most powerful domains.   Some of the troops were also from the common classes, farmers or craftsmen, who were conscripted to fight. In addition, Japanese workers built a huge naval base on western Kyushu, just across the Tsushima Strait from Korea. The naval force that would ferry this enormous army across the strait consisted of both men-of-war and requisitioned pirate boats, manned by a total of 9,000 sailors. Japan Attacks The first wave of Japanese troops arrived at Busan, on Koreas southeast corner, on April 13, 1592. Some 700 boats offloaded three divisions of samurai soldiers, who rushed Busans unprepared defenses and captured this major port in a matter of hours. The few Korean soldiers who survived the onslaught sent messengers running to King Seonjos court in Seoul, while the rest retreated inland to try to regroup. Armed with muskets, against Koreans with bows and swords, the Japanese troops quickly swept toward Seoul. About 100 kilometers from their target, they met the first real resistance on April 28 - a Korean army of about 100,000 men at Chungju. Not trusting his green recruits to stay on the field, Korean general Shin Rip staged his forces in a swampy y-shaped area between the Han and Talcheon Rivers.   The Koreans had to stand and fight or die.   Unfortunately for them, the 8,000 Korean cavalry riders bogged down in flooded rice paddies and Korean arrows had a much shorter range than the Japanese muskets. The Battle of Chungju soon turned into a massacre. General Shin led two charges against the Japanese, but couldnt break through their lines. Panicking, the Korean troops fled and jumped into the rivers where they drowned, or got hacked down and decapitated by samurai swords.   General Shin and the other officers committed suicide by drowning themselves in the Han River. When King Seonjo heard that his army was destroyed, and the hero of the Jurchen Wars, General Shin Rip, was dead, he packed up his court and fled north. Angry that their king was deserting them, people along his flight path stole all of the horses from the royal party. Seonjo didnt stop until he reached Uiju, on the Yalu River, which is now the border between North Korea and China. Just three weeks after they landed at Busan, the Japanese captured the Korean capital of Seoul (then called Hanseong). It was a grim moment for Korea. Admiral Yi and the Turtle Ship Unlike King Seonjo and the army commanders, the admiral who was in charge of defending Koreas southwest coast had taken the threat of a Japanese invasion seriously, and had begun to prepare for it.   Admiral Yi Sun-shin, the Left Navy Commander of Cholla Province, had spent the previous couple of years building up Koreas naval strength.   He even invented a new kind of ship unlike anything known before. This new ship was called the kobuk-son, or turtle ship, and it was the worlds first iron-clad warship. The kobuk-sons deck was covered with hexagonal iron plates, as was the hull, to prevent enemy cannon shot from damaging the planking and to ward off fire from flaming arrows. It had 20 oars, for maneuverability and speed in battle. On the deck, iron spikes jutted up to discourage boarding attempts by enemy fighters. A dragons head figurehead on the bow concealed four cannon that fired iron shrapnel at the enemy. Historians believe that Yi Sun-shin himself was responsible for this innovative design. With a much smaller fleet than Japans, Admiral Yi racked up 10 crushing naval victories in a row through use of his turtle ships, and his brilliant battle tactics. In the first six battles, the Japanese lost 114 ships and many hundreds of their sailors. Korea, in contrast, lost zero ships and 11 sailors. In part, this amazing record was also due to the fact that most of Japans sailors were poorly-trained former pirates, while Admiral Yi had been carefully training a professional naval force for years. The Korean Navys tenth victory brought Admiral Yi an appointment as the Commander of the Three Southern Provinces. On July 8, 1592, Japan suffered its worst defeat yet at the hands of Admiral Yi and the Korean navy. In the Battle of Hansan-do, Admiral Yis fleet of 56 met a Japanese fleet of 73 ships. The Koreans managed to encircle the larger fleet, destroying 47 of them and capturing 12 more. Approximately 9,000 Japanese soldiers and sailors were killed.   Korean lost none of its ships, and just 19 Korean sailors died. Admiral Yis victories at sea were not simply an embarrassment for Japan. The Korean naval actions cut off the Japanese army from the home islands, leaving it stranded in the middle of Korea without supplies, reinforcements, or a communication route. Although the Japanese were able to capture the old northern capital at Pyongyang on July 20, 1592, their northward movement soon bogged down.   Rebels and Ming With the tattered remnants of the Korean army hard-pressed, but filled with hope thanks to Koreas naval victories, the ordinary people of Korea rose up and began a guerrilla war against the Japanese invaders. Tens of thousands of farmers and slaves picked off small groups of Japanese soldiers, set fire to Japanese camps, and generally harried the invading force in every possible way. By the end of the invasion, they were organizing themselves into formidable fighting forces, and winning set battles against the samurai. In February, 1593, the Ming government finally realized that the Japanese invasion of Korea posed a serious threat to China as well. By this time, some Japanese divisions were battling with the Jurchens in what is now Manchuria, northern China. The Ming sent an army of 50,000 which quickly routed the Japanese from Pyongyang, pushing them south to Seoul.   Japan Retreats China threatened to send a much larger force, some 400,000 strong, if the Japanese didnt withdraw from Korea. The Japanese generals on the ground agreed to withdraw to the area around Busan while peace talks were held. By May of 1593, most of the Korean Peninsula had been liberated, and the Japanese were all concentrated in a narrow coastal strip on the southwestern corner of the country. Japan and China chose to hold peace talks without inviting any Koreans to the table. In the end, these would drag on for four years, and emissaries for both sides brought false reports back to their rulers. Hideyoshis generals, who feared his increasingly erratic behavior and his habit of having people boiled alive, gave him the impression that they had won the Imjin War. As a result, Hideyoshi issued a series of demands: China would allow Japan to annex the four southern provinces of Korea; one of the Chinese emperors daughters would be married to the Japanese emperors son; and Japan would receive a Korean prince and other nobles as hostages to guarantee Koreas compliance with Japanese demands. The Chinese delegation feared for their own lives if they presented such an outrageous treaty to the Wanli Emperor, so they forged a much more humble letter in which Hideyoshi begged China to accept Japan as a tributary state. Predictably,  Hideyoshi was incensed  when the Chinese emperor replied to this forgery late in 1596 by granting Hideyoshi the bogus title King of Japan, and giving Japan status as a vassal state of China. The Japanese leader ordered preparations for a second invasion of Korea. Second Invasion On August 27, 1597, Hideyoshi sent an armada of 1000 ships carrying 100,000 troops to reinforce the 50,000 who remained at Busan. This invasion had a more modest goal - simply to occupy Korea, rather than to conquer China. However, the Korean army was much better prepared this time, and the Japanese invaders had a tough slog ahead of them. The second round of the Imjin War also began with a novelty - the Japanese navy defeated the Korean navy at the Battle of Chilcheollyang, in which all but 13 Korean ships were destroyed. In large part, this defeat was due to the fact that Admiral Yi Sun-shin had been the victim of a whispered smear campaign at court, and had been removed from his command and imprisoned by King Seonjo.  After the disaster of Chilcheollyang, the king quickly pardoned and reinstated Admiral Yi.   Ã‚   Japan planned to seize the entire southern coast of Korea, then march for Seoul once more. This time, however, they met a joint Joseon and Ming army at Jiksan (now Cheonan), which held them off from the capital and even began to push them back toward Busan. Meanwhile, the reinstated Admiral Yi Sun-shin led the Korean navy in its most astonishing victory yet at the Battle of Myongnyang in October of 1597. The Koreans were still trying to rebuild after the Chilcheollyang fiasco; Admiral Yi had just 12 ships under his command.   He managed to lure 133 Japanese vessels in to a narrow channel, where the Korean ships, strong currents, and rocky coastline destroyed them all. Unbeknownst to the Japanese troops and sailors, Toyotomi Hideyoshi had died back in Japan on September 18, 1598. With him died all will to continue this grinding, pointless war. Three months after the warlords death, the Japanese leadership ordered a general retreat from Korea. As the Japanese began to withdraw, the two navies fought one last great battle at the Noryang Sea. Tragically, in the midst of another stunning victory, Admiral Yi was hit by a stray Japanese bullet and died on the deck of his flagship.   In the end, Korea lost an estimated 1 million soldiers and civilians in the two invasions, while Japan lost more than 100,000 troops. It was a senseless war, but it did give Korea a great national hero and a new naval technology - the famous turtle ship.

The Crucible By Arthur Miller - 1235 Words

â€Å"Your reputation and integrity are everything. Follow through on what you say you’re going to do. Your credibility can only be built over time, and it is built from the history of your words and actions.† Maria Razumich-Zec put into words the importance that society places on reputation. It has become clear that what other people think of someone is far more important to that individual than what they may know to be true about themselves. In todays society is very rare to find people that are content with what they are, despite what the people surrounding them believe. This notion that in order to be validated there has to be external approval dates back to the seventeenth century and earlier. During the Salem Witch Trials, men and women were killed daily due to the thoughts of others. In Arthur Miller’s Crucible, it is clearly demonstrated that reputation is a huge role in the daily lives of people. A fine example of how The Crucible displays the importance of reputation is obviously with the character of John Proctor. The actions that take place in the play are due to Abigail Williams longing for John Proctor to be hers yet again. John and Abigail had an affair months back, and in fear of what may happen to her family, Elizabeth Proctor banished Abigail from her home. During this time, the act of adultery was a heinous crime. If one were to commit such a sin, then the punishment would be severe. In fear of the consequences he might face, even just as simple as theShow MoreRelatedThe Crucible By Arthur Miller1269 Words   |  6 PagesAt first glance, the playwright Arthur Miller in The Crucible highlights the historical significance of the Salem Witch Trials of 1692, but in fact it is an allegorical expression of his perception of McCarthyism. If the reader has some background information on Arthur Miller’s victimization as a communist, it is evident that the play is a didactic vessel illustrating the flaws of the court syste m in the 1950’s. The communist allegations were launched at government employees, entertainers and writersRead MoreThe Crucible By Arthur Miller1681 Words   |  7 Pagesof their way to the last dying breath to make sure they leave with a good or bad reputation. In one of the recent literature study in class â€Å"The Crucible† by Arthur Miller, Miller uses characterization to illustrate reputation throughout the play. â€Å"The Crucible† takes place in Salem, Massachusetts. It is based upon the Salem witch trails. In â€Å"The Crucible†, we journey through the life of three characters who reputations plays a major role in the play. The three characters are John Proctor, AbigailRead MoreThe Crucible By Arthur Miller998 Words   |  4 Pagesmotivated by jealousy and spite. The Crucible is a four-act dramatic play production that was first performed on January 22, 1953. Arthur Miller used dialogue within the characters to cover the multiple themes; conflicts and resolutions, plus the few directions for the different actions of the play. The Salem Witch Trials were intended to be performed as the play however, when read, it can be more carefully examined and broken down to analyze the techniques. Miller, the playwright, uses literaryRead MoreThe Crucible By Arthur Miller1333 Words   |  6 PagesAs the various characters in The Crucible by Arthur Miller interact, the dominant theme of the consequences of women’s nonconformity begins to slide out from behind the curtains of the play. Such a theme reveals the gripping fear that inundated the Puritans during the seventeenth century. This fear led to the famous witch-hunts that primarily terrorized women who deviated from the Puritan vision of absolute obedience and orthodoxy. Arthur Miller presents his interpretation of the suffering by subtlyRead MoreThe Crucible By Arthur Miller1145 Words   |  5 PagesUnbalance Through The Centuries In Arthur Miller’s play, The Crucible, the author reflects the persecution of communists in America in the 1950’s through a recount of the Salem witch trials. It is often presumed that Miller based his drama directly off of events that were particularly prevalent in the years surrounding the publication of The Crucible- which was released in the year 1953, towards the conclusion of the Korean War. Although there was not a literal witch hunt occurring during this timeRead MoreThe Crucible By Arthur Miller1063 Words   |  5 PagesIn the English dictionary, there are three definitions of the word crucible. One is a metal container in which metals are mixed and melted. Another is a severe test. But the third definition, and the one that I think fits the best for this book, is a place or situation in which different elements interact to create something new. In my mind, this fits because all of the characters had their little grudges and dirty secrets. But when all th ose seemingly little things interact, they formed somethingRead MoreThe Crucible By Arthur Miller1285 Words   |  6 Pages Rationale, Morality, Stereotypes, Pressure, Self-Censorship, Unanimity, and Mindguards. Groupthink has also taken place in our history a a country. The play, The Crucible by Arthur Miller is about a the real-life Salem Witch Trials that happened in 1692 - 1693, in Salem, Massachusetts. Some symptoms of Groupthink found in the Crucible are Rationale, Pressure, and Self-Censorship. The Groupthink symptom, Rationale, is described as when victims of Groupthink ignore warnings: they also collectivelyRead MoreThe Crucible By Arthur Miller841 Words   |  4 PagesThe Crucible is a chaotic play, throughout this American classic Arthur Miller takes the reader through multiple events of terror and insanity. While creating a great on-stage play, Arthur Miller portrays his life through the events, the characters, and plot of The Crucible. Using vivid imagery and comprehensible symbolism, Miller manipulates the real personalities of the characters and events in 1600 Salem, Massachusetts to create a symbolic autobiography. Throughout this play, the reader experiencesRead MoreThe Crucible By Arthur Miller811 Words   |  4 Pages While The Crucible, by Arthur Miller, is only a four act play, it still resembles the format of a five act play. The five-act structure evolved from a three-act structure, which was made famous by Roman Aelius Donatus. Donatus came up with thre e types of plays: Protasis, Epitasis, and Catastrophe. The five-act structure helped to expand the three act structure, mainly made famous by Shakespeare through his many tragedies. Even though The Crucible contains only four acts, it still has the commonRead MoreThe Crucible By Arthur Miller1052 Words   |  5 PagesBuddy Al-Aydi Ms.Healy English 9 CP 14th October 2014 The Crucible Essay The Crucible was a novel written by Arthur Miller in the 1950’s. It was written in a format of the play, portraying an allegory of the Salem Witch-Hunts led by Senator Joseph McCarthy. The book is known to have a inexplicable plot. This plot is advanced by multiple characters in the book in order to ensure that the reader maintains interest with the material that is being read. The farmer, John Proctor, would be the

Biological Function of CRISPR Technology

Question: Discuss about the Biological Function of CRISPR Technology. Answer: Introduction: Clustered regularly interspaced short palindromic repeats (CRISPR) are prokaryotic DNA segments with short and repetitive base sequences. (Cong et al. 2013). The actual biological function of CRISPR and Cas genes is seen in adaptive immunity of archaea and selected bacteria. With the help of these genes, the organisms are able to have an appropriate response to the genetic material and eliminate it consequently. There are three kinds of CRISPR mechanisms, among which the type II is mostly analyzed by researchers. In type II, the foreign DNA is suitably cut into smaller parts and entered into the CRISPR locus within a series of short repeats. These repeats are of 20 base pairs. The loci are then transcribed and transcripts are subsequently processed for the generation of small RNAs. These RNAs are used for guiding effector endonucleases targeting DNA on the basis of sequence complementarity. Cas9 protein, a kind of Cas protein, plays a significant role in CRISPR mechanisms. This Cas9 protein plays a role in gene silencing. It takes part in the processing of crRNA and also plays a role in the destruction of the DNA that is made the target. The function of the Cas9 in two steps is dependent on the action of two nuclease domains. One of these is the RuvC-like nuclease domain that is located at the amino terminus and the second is the HNH-like nuclease domain whose location is in the mid-region of the protein. For achieving the site-specific recognition and cleavage of the DNA, Cas9 has to form a complex with the crRNA as well as with the separate trans-activating crRNA. The trans-activating crRNA and the crRNA are partially complementary. For the maturation of crRNA from a primary transcript encoding, there is a requirement for the tracrRNA. The maturation leads to the encoding of multiple pre-crRNAs. The presence of Cas9 and RNase III is needed in this regard. At the time of the destruction of DNA that is made the target, the RuvC-like and HNH nuclease domains have to cut two of the DNA strands, as a result of which double-stranded breaks (DSBs) are generated at location where 20-nucleotide target sequence are present in linked crRNA transcript. The RuvC domain plays a role in cleaving the non-complimentary strand whereas the HNH domain function is to cleave the complementary strand. The double-stranded endonuclease activity exhibited by the Cas9 also needs the presence of a short conserved sequence, (25 nts) known as the protospacer-associated motif (PAM). It needs to follow immediately the 3- of the crRNA complementary sequence. As a matter of fact, if PAM is absent then Cas9-RNA does not recognise the fully complementary sequences. The plainness of the type II CRISPR nuclease, as it requires only three components (Cas9, crRNA and trRNA) makes the system acquiescent to alteration for editing of genome (Hsu, Lander and Zhang, 2014). The CRISPR method is being used for the introduction of single point mutations, insertions or deletions, in a certain gene that is targeted through the help of single gRNA (Guide-RNA). With the utilisation of gRNA-directed Cas9 nuclease, it is also possible to delete large genomic rearrangements, like translocations and inversions. The latest development is the application of a dCas9 version of the CRISPR/Cas9 system to protein domains that are made the target for regulation of transcription, epigenetic modification and visualisation of the specific genome loci. The CRISPR/Cas9 system needs a redesigning in the crRNA for changing target specificity. This is in contrast with genome editing tools, like zinc finger and TALENs, that needs redesigning of the protein-DNA interface (Zhang et al. 2015). References Cong, L., Ran, F.A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P.D., Wu, X., Jiang, W., Marraffini, L.A. and Zhang, F., 2013. Multiplex genome engineering using CRISPR/Cas systems.Science,339(6121), pp.819-823. Hsu, P.D., Lander, E.S. and Zhang, F., 2014. Development and applications of CRISPR-Cas9 for genome engineering.Cell,157(6), pp.1262-1278. Peel, N. (2017).CRISPR gene editing: new chapter in cancer research or blot in the ethical copybook?. [online] Cancer Research UK - Science blog. Available at: https://scienceblog.cancerresearchuk.org/2016/02/01/crispr-gene-editing-new-chapter-in-cancer-research-or-blot-in-the-ethical-copybook/ [Accessed 19 Mar. 2017]. Zhang, Y., Yeo, W.S., Ng, K.H. and Yap, H.K., 2015. CRISPR/Cas9 for Genome Engineering: the Next Genomic Revolution.Journal of Biochemistry and Molecular Biology Research,1(4), pp.112-117.